| MicroRNA快速检测系统的设计 |
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| 引用本文： | 王燕飞,余东升,陈海燕,张战盈,方蔚恺,陆泽橼,李艳蕾,纪玉峰,关一夫,徐赤东,江海河. MicroRNA快速检测系统的设计[J]. 光子学报, 2017, 46(3). DOI: 10.3788/gzxb20174603.0312001 |
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| 作者姓名： | 王燕飞 余东升 陈海燕 张战盈 方蔚恺 陆泽橼 李艳蕾 纪玉峰 关一夫 徐赤东 江海河 |
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| 作者单位： | 1. 中国科学院合肥物质科学研究院医学物理与技术中心,合肥230031;中国科学技术大学,合肥230026;2. 中国科学院合肥物质科学研究院医学物理与技术中心,合肥,230031;3. 中国科学院合肥物质科学研究院医学物理与技术中心,合肥230031;中国医科大学,沈阳110001 |
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| 基金项目： | 国家自然科学基金,安徽省自然科学基金(No.1508085SMC214)资助 The National Natural Science Foundation of China,Anhui Provincial Natural Science Foundation |
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| 摘 要： | 为了实现MicroRNA的快速检测,设计了一种便携式MicroRNA快速检测仪.基于等温滚环扩增技术,采用光电检测方法,检测标志物受激发出的荧光光强,建立特征荧光分析检测系统.通过改变激发光强度、MicroRNA试剂浓度等参量,验证了该仪器可测量的MicroRNA的浓度范围为0.01~0.1μmol,可检测出的最低检出限为7个拷贝数,MicroRNA浓度与荧光信号强度之间为线性关系(R2=0.999 1).
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| 关 键 词： | 生物医学工程 医用光学仪器 核酸检测 荧光 弱光探测 MicroRNA 滚环扩增 |
Design of a Rapid MicroRNA Detection System |
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| WANG Yan-fei,YU Dong-sheng,CHEN Hai-yan,ZHANG Zhan-ying,FANG Wei-kai,LU Ze-yuan,LI Yan-lei,JI Yu-feng,GUAN Yi-fu,XU Chi-dong,JIANG Hai-he. Design of a Rapid MicroRNA Detection System[J]. Acta Photonica Sinica, 2017, 46(3). DOI: 10.3788/gzxb20174603.0312001 |
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| Authors: | WANG Yan-fei YU Dong-sheng CHEN Hai-yan ZHANG Zhan-ying FANG Wei-kai LU Ze-yuan LI Yan-lei JI Yu-feng GUAN Yi-fu XU Chi-dong JIANG Hai-he |
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| Abstract: | Using Opto-electronic detection technology,marking miRNA and isothermal rolling circle amplification and detecting markers which excite fluorescence intensity,we have designed a new portable device for the rapid detection of miRNA,the recognition system of characteristic fluorescence analysis was established eventually,we have got the point that there is the linear relationship (R2 =0.999 1) between miRNA concentration and fluorescence intensity at the time that the miRNA concentration range the instrument can measure is in the range of 0.01～0.1 μmol by changing the excitation light inten sity,miRNA reagent concentration and other parameters,meanwhile,we also know that it is 7 copies that the lowest values the instrument can measure in theory. |
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| Keywords: | Biomedical engineering Medical optics instrumentation Nucleic acid detection Fluorescence Light detection MicroRNA Rolling circle amplification |
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