Micro-Lensed Fiber Laser Desorption Mass Spectrometry Imaging Reveals Subcellular Distribution of Drugs within Single Cells |
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Authors: | Dr. Yifan Meng Xiaoling Cheng Tongtong Wang Prof. Wei Hang Xiaoping Li Wan Nie Rong Liu Zheng Lin Le Hang Zhibin Yin Prof. Baolin Zhang Prof. Xiaomei Yan |
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Affiliation: | 1. Ministry of Education (MOE) Key Laboratory of Spectrochemical Analysis and Instrumentation, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005 China;2. State Key Laboratory Breeding Base of Nonferrous Metals and Specific Materials Processing, College of Materials Science and Engineering, Guilin University of Technology, Guilin, 541004 China |
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Abstract: | The visualization of temporal and spatial changes in the intracellular environment has great significance for chemistry and bioscience research. Mass spectrometry imaging (MSI) plays an important role because of its unique advantages, such as being label-free and high throughput, yet it is a challenge for laser-based techniques due to limited lateral resolution. Here, we develop a simple, reliable, and economic nanoscale MSI approach by introducing desorption laser with a micro-lensed fiber. Using this integrated platform, we achieved 300 nm resolution MSI and successfully visualized the distribution of various small-molecule drugs in subcellular locations. Exhaustive dynamic processes of anticancer drugs, including releasing from nanoparticle carriers entering nucleus of cells, can be readily acquired on an organelle scale. Considering the simplicity and universality of this nanoscale desorption device, it could be easily adapted to most of laser-based mass spectrometry applications. |
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Keywords: | laser-based techniques mass spectrometry imaging micro-lensed fiber single-cell imaging subcellular resolution |
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