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Cross-Link-Functionalized Nanoparticles for Rapid Excretion in Nanotheranostic Applications |
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| Authors: | Zhuoran Ma Dr Feifei Wang Dr Yeteng Zhong Felix Salazar Jiachen Li Prof Mingxi Zhang Dr Fuqiang Ren Prof Anna M Wu Prof Hongjie Dai |
| Institution: | 1. Department of Chemistry and Bio-X, Stanford University, Stanford, CA, 94305 USA;2. Department of Chemistry and Bio-X, Stanford University, Stanford, CA, 94305 USA
These authors contributed equally to this work.;3. Department of Molecular Imaging and Therapy, Beckman Research Institute of the City of Hope, Duarte, CA, USA;4. State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology, Wuhan, China |
| Abstract: | Most NIR-IIb fluorophores are nanoparticle-based probes with long retention (≈1 month or longer) in the body. Here, we applied a novel cross-linked coating to functionalize core/shell lead sulfide/cadmium sulfide quantum dots (PbS/CdS QDs) emitting at ≈1600 nm. The coating was comprised of an amphiphilic polymer followed by three crosslinked amphiphilic polymeric layers (P3 coating), imparting high biocompatibility and >90 % excretion of QDs within 2 weeks of intravenous administration. The P3-QDs were conjugated to an engineered anti-CD8 diabody (Cys-diabody) for in vivo molecular imaging of CD8+ cytotoxic T lymphocytes (CTLs) in response to anti-PD-L1 therapy. Two-plex molecular imaging in combination with down-conversion Er nanoparticles (ErNPs) was performed for real-time in vivo monitoring of PD-L1 positive tumor cells and CTLs with cellular resolution by non-invasive NIR-IIb light sheet microscopy. Imaging of angiogenesis in the tumor microenvironment and of lymph nodes deep in the body with a signal-to-background ratio of up to ≈170 was also achieved using P3-QDs. |
| Keywords: | excretion imaging agents nanomedicine nanoparticles second near-infrared window |