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Integrating Time-Resolved Imaging Information by Single-Luminophore Dual Thermally Activated Delayed Fluorescence |
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| Authors: | Mengkai Luo Dr. Xuping Li Longjiang Ding Dr. Gleb Baryshnikov Shen Shen Dr. Mingjie Zhu Dr. Lulu Zhou Dr. Man Zhang Prof. Dr. Jianjun Lu Prof. Dr. Hans Ågren Prof. Dr. Xu-dong Wang Prof. Dr. Liangliang Zhu |
| Affiliation: | 1. State Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science, Fudan University, Shanghai, 200438 China These authors contributed equally to this work.;2. State Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science, Fudan University, Shanghai, 200438 China Key Laboratory of Coal Science and Technology, Ministry of Education and Shanxi Province, Taiyuan University of Technology, Taiyuan, 030024 China These authors contributed equally to this work.;3. Department of Chemistry, Fudan University, Shanghai, 200438 China;4. Division of Theoretical Chemistry and Biology School of Biotechnology, KTH Royal Institute of Technology, 10691 Stockholm, Sweden;5. State Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science, Fudan University, Shanghai, 200438 China;6. Key Laboratory of Coal Science and Technology, Ministry of Education and Shanxi Province, Taiyuan University of Technology, Taiyuan, 030024 China |
| Abstract: | The fact that the lifetime of photoluminescence is often difficult to access because of the weakness of the emission signals, seriously limits the possibility to gain local bioimaging information in time-resolved luminescence probing. We aim to provide a solution to this problem by creating a general photophysical strategy based on the use of molecular probes designed for single-luminophore dual thermally activated delayed fluorescence (TADF). The structural and conformational design makes the dual TADF strong in both diluted solution and in an aggregated state, thereby reducing sensitivity to oxygen quenching and enabling a unique dual-channel time-resolved imaging capability. As the two TADF signals show mutual complementarity during probing, a dual-channel means that lifetime mapping is established to reduce the time-resolved imaging distortion by 30–40 %. Consequently, the leading intracellular local imaging information is serialized and integrated, which allows comparison to any single time-resolved signal, and leads to a significant improvement of the probing capacity. |
| Keywords: | aggregated state anti-Kasha's rule bioimaging dual emissions thermally activated delayed fluorescence |