Simultaneous Detection of Carbon Monoxide and Viscosity Changes in Cells |
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| Authors: | Dr Jonathan A Robson Dr Markéta Kubánková Tamzin Bond Rian A Hendley Dr Andrew J P White Prof?Dr Marina K Kuimova Prof?Dr James D E T Wilton-Ely |
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| Institution: | Department of Chemistry, Molecular Sciences Research Hub, Imperial College London, White City Campus, London, W12 0BZ UK |
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| Abstract: | A new family of robust, non-toxic, water-compatible ruthenium(II) vinyl probes allows the rapid, selective and sensitive detection of endogenous carbon monoxide (CO) in live mammalian cells under normoxic and hypoxic conditions. Uniquely, these probes incorporate a viscosity-sensitive BODIPY fluorophore that allows the measurement of microscopic viscosity in live cells via fluorescence lifetime imaging microscopy (FLIM) while also monitoring CO levels. This is the first example of a probe that can simultaneously detect CO alongside small viscosity changes in organelles of live cells. |
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| Keywords: | endogenous CO fluorescence ruthenium sensing viscosity |
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