| Affiliation: | 1. Nano-Bio Translational Research Laboratory, Department of Biotechnology, Bannari Amman Institute of Technology, Sathyamangalam, Erode, Tamil Nadu, 638 401 India;2. Department of Microbiology and Immunology, Division of Biomedical Sciences, School of Medicine, College of Health Sciences, Mekelle University, Mekelle, 1871 Ethiopia;3. Department of Physics, School of Advanced Sciences, Vellore Institute of Technology, Vellore, 632 014 India Institute of Physics, Academia Sinica, Taipei, Taiwan;4. Food Technology, Engineering and Nutrition, Lund University, PO Box 124, Lund, SE-221 00 Sweden;5. Department of Biotechnology, National Institute of Technology, Tadepalligudem, Andhra Pradesh, 534 101 India |
| Abstract: | This study is an attempt to improve the loading and release potential of silver oxide nanoparticles (AgO NPs) using Oleic acid based bio-surfactant isolated from Enteromorpha instestinalis. The isolated Oleic acid based bio-surfactant was characterized using GC–MS, NMR, and HPLC. The AgO NPs were then surface-functionalized with the bio-surfactant and analyzed using XRD, HR-TEM/SAED, DLS, UV–Vis, and FTIR spectroscopy to evaluate the loading of aspirin and functional groups responsible for loading. The model drug Aspirin that has depreciated bio-availability due to poor solubility was used to test the drug carrier efficiency of the AgO NPs after the surface-functionalization. The loading of aspirin was increased by up to 80% in bio-surfactants than PEG-coated NPs (72%) at a 1:1 ratio (Aspirin/NP). The drug release profile of aspirin was evaluated by dialysis at different acidic conditions (pH 1.2, 6.8, and 7.4) and the active release of aspirin was observed in pH 6.8 and bio-surfactant produced better release than PEG and commercial tablet in all the pH conditions. To identify the mechanism of release from the carrier, the release kinetics was studied using zero-order, first-order, Higuchi's, and Korsmeyer Peppas equations and found that the release was time-dependent and non-fickian. |
