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J-Aggregate-Based FRET Monitoring of Drug Release from Polymer Nanoparticles with High Drug Loading |
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| Authors: | Yun Liu Guangze Yang Song Jin Run Zhang Peng Chen Tengjisi Lianzhou Wang Dong Chen David A Weitz Chun-Xia Zhao |
| Institution: | 1. Australian Institute for Bioengineering and Nanotechnology, University of Queensland, St. Lucia, Queensland, 4072 Australia
These authors contributed equally to this work.;2. Australian Institute for Bioengineering and Nanotechnology, University of Queensland, St. Lucia, Queensland, 4072 Australia;3. Nanomaterials Centre, School of Chemical Engineering, Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, QLD, 4072 Australia;4. Institute of Process Equipment, College of Energy Engineering, Zhejiang University, Hangzhou, Zhejiang, 310027 China;5. John A. Paulson School of Engineering and Applied Sciences, Harvard University, Cambridge, MA, 02138 USA |
| Abstract: | Understanding drug-release kinetics is critical for the development of drug-loaded nanoparticles. We developed a J-aggregate-based Förster-resonance energy-transfer (FRET) method to investigate the release of novel high-drug-loading (50 wt %) nanoparticles in comparison with low-drug-loading (0.5 wt %) nanoparticles. Single-dye-loaded nanoparticles form J-aggregates because of the high dye-loading (50 wt %), resulting in a large red-shift (≈110 nm) in the fluorescence spectrum. Dual-dye-loaded nanoparticles with high dye-loading using FRET pairs exhibited not only FRET but also a J-aggregate red-shift (116 nm). Using this J-aggregate-based FRET method, dye-core–polymer-shell nanoparticles showed two release processes intracellularly: the dissolution of the dye aggregates into dye molecules and the release of the dye molecules from the polymer shell. Also, the high-dye-loading nanoparticles (50 wt %) exhibited a slow release kinetics in serum and relatively quick release in cells, demonstrating their great potential in drug delivery. |
| Keywords: | drug release FRET J-aggregates nanoparticles polymers |