An Electrochemophysiological Microarray for Real-Time Monitoring and Quantification of Multiple Ions in the Brain of a Freely Moving Rat |
| |
Authors: | Fan Zhao Yuandong Liu Hui Dong Shiqing Feng Prof. Guoyue Shi Longnian Lin Prof. Yang Tian |
| |
Affiliation: | 1. Shanghai Key Laboratory of Green Chemistry and Chemical Processes, East China Normal University, Dongchuan Road 500, Shanghai, 200092 China;2. Key Laboratory of Brain Functional Genomics-Ministry of Education, School of Life Science Department, East China Normal University, North Zhongshan Road, Shanghai, 200241 China |
| |
Abstract: | Herein, we present an electrochemophysiological microarray (ECPM) for real-time mapping and simultaneous quantification of chemical signals for multiple ions in the deep brain of a freely moving rat, in which microelectrode arrays were developed for direct determination of multiple ions using open-circuit potentiometry. Specific recognition ionophores were synthesized and optimized for determination of K+, Ca2+, Na+ and pH. A reference electrode was also developed to avoid interferences in the brain. The microarrays were successfully applied in real-time monitoring and quantification of ions in a live brain. The extra current-free potentiometry allowed mapping and biosensing of chemical signals, together with recording of electrical signals in the whole brain without cross-talk, for the first time. Furthermore, the ECPM provided a platform for real-time monitoring of the dynamic changes of multiple ions in the deep brain of freely moving rat during a seizure. |
| |
Keywords: | brain electrochemistry freely moving rats ion channels microelectrode arrays |
|
|