Aptamer-Mediated Reversible Transactivation of Gene Expression by Light |
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Authors: | Christian Renzl Dr Ankana Kakoti Prof Dr Günter Mayer |
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Institution: | LIMES, University of Bonn, Gerhard-Domagk-Str. 1, 53121 Bonn, Germany |
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Abstract: | The investigation and manipulation of cellular processes with subcellular resolution requires non-invasive tools with spatiotemporal precision and reversibility. Building on the interaction of the photoreceptor PAL with an RNA aptamer, we describe a variation of the CRISPR/dCAS9 system for light-controlled activation of gene expression. This platform significantly reduces the coding space required for genetic manipulation and provides a strong on-switch with almost no residual activity in the dark. It adds to the current set of modular building blocks for synthetic biological circuit design and is broadly applicable. |
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Keywords: | aptamers gene expression synthetic biology |
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