Catalytically Active Hollow Fiber Membranes with Enzyme-Embedded Metal–Organic Framework Coating |
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Authors: | Daniel Josef Bell Monika Wiese Ariel Augusto Schönberger Matthias Wessling |
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Institution: | Chemical Process Engineering, RWTH Aachen University, Forckenbeckstr. 51, 52074 Aachen, Germany |
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Abstract: | Metal–organic frameworks (MOFs) are suitable enzyme immobilization matrices. Reported here is the in situ biomineralization of glucose oxidase (GOD) into MOF crystals (ZIF-8) by interfacial crystallization. This method is effective for the selective coating of porous polyethersulfone microfiltration hollow fibers on the shell side in a straightforward one-step process. MOF layers with a thickness of 8 μm were synthesized, and fluorescence microscopy and a colorimetric protein assay revealed the successful inclusion of GOD into the ZIF-8 layer with an enzyme concentration of 29±3 μg cm?2. Enzymatic activity tests revealed that 50 % of the enzyme activity is preserved. Continuous enzymatic reactions, by the permeation of β-d -glucose through the GOD@ZIF-8 membranes, showed a 50 % increased activity compared to batch experiments, emphasizing the importance of the convective transport of educts and products to and from the enzymatic active centers. |
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Keywords: | enzymes membranes metal–organic frameworks oxidation polymers |
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