An unexpected fluorescent probe for G-quadruplex DNA based on a nitro-substituted ruthenium (II) complex |
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| Authors: | Xue-Wen Liu Ning-Yi Liu Yuan-Qing Deng Shan Wang Ting Liu Yu-Cai Tang Yuan-Dao Chen Ji-Lin Lu |
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| Institution: | 1. Hunan Province Cooperative Innovation Center for the Construction & Development of Dongting Lake Ecological Economic Zone, Hunan Provincial Key Laboratory of Water Treatment Functional Materials, Hunan Province Engineering Research Center of Electroplating Wastewater Reuse Technology, College of Chemistry and Materials Engineering, Hunan University of Arts and Science, Changde, 415000 China;2. Hunan Province Cooperative Innovation Center for the Construction & Development of Dongting Lake Ecological Economic Zone, Hunan Provincial Key Laboratory of Water Treatment Functional Materials, Hunan Province Engineering Research Center of Electroplating Wastewater Reuse Technology, College of Chemistry and Materials Engineering, Hunan University of Arts and Science, Changde, 415000 China
State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, 210023 P. R. China |
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| Abstract: | The detection of G-quadruplex is of major interest. Nitro-substituted ruthenium (II) complexes have attracted much attention due to fluorescent sensitivity to environment change. We report here a new nitro-substituted ruthenium (II) complex, Ru (phen)2(hnoip)]2+ ( 1) (hnoip = 2-(2-hydroxyl-5-nitrophenyl)imidazo4,5-f]1,10-phenanthroline]), which displays distinct fluorescent properties in aqueous solution and non-aqueous solvents. This complex exhibits large fluorescence enhancement after binding with G-quadruplex DNA, and displays good fluorescent selectivity over other DNAs. The limit of detection is 6 nm for 22AG in Na+ and 43 nm for 22AG in K+, respectively. The results demonstrated that nitro-substituted ruthenium (II) complexes can be utilized to design as G-quadruplex fluorescent probes by protection of the nitro group on the complex from water. |
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| Keywords: | fluorescent probe G-quadruplex DNA nitro group ruthenium complex |
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