Simple surface functionalization of magnetic nanoparticles with methotrexate-conjugated bovine serum albumin as a biocompatible drug delivery vehicle |
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Authors: | Sedigheh Azizi Hamed Nosrati Hossein Danafar |
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Affiliation: | Zanjan Pharmaceutical Nanotechnology Research Center, Zanjan University of Medical Sciences, Zanjan, Iran |
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Abstract: | Magnetic nanoparticles (MNPs) functionalized with methotrexate (MTX)-conjugated bovine serum albumin (BSA) as a biocompatible drug delivery vehicle were synthesized using a facile method. Characterization of the functionalized MNPs (Fe3O4@BSA-MTX NPs) was performed using various techniques including UV–visible spectroscopy, dynamic light scattering, vibrating sample magnetometry and X-ray diffraction. The particle size and zeta potential of Fe3O4@BSA-MTX NPs were 105.7 ± 3.81 nm (mean ± SD) and −18.2 mV, respectively. MTX release from Fe3O4@BSA-MTX NPs showed an enzyme-dependent release pattern. Hemo-biocompatibility of Fe3O4@BSA-MTX NPs was confirmed using hemolysis test. In addition, the cytotoxicity of functionalized MNPs and free MTX against MCF-7 cell line was investigated using MTT assay. The results of experiments revealed that the Fe3O4@BSA-MTX NPs as a biocompatible carrier could improve the therapeutic effect of MTX. |
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Keywords: | bovine serum albumin (BSA) enzyme-dependent release magnetic nanoparticles methotrexate (MTX) protein-based vehicle |
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