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Sequence-Dependent DNA Functionalization of Upconversion Nanoparticles and Their Programmable Assemblies |
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| Authors: | Huan Ge Dongya Wang Yue Pan Yuanyuan Guo Hongyu Li Fan Zhang Prof. Xinyuan Zhu Prof. Yuehua Li Prof. Chuan Zhang Prof. Ling Huang |
| Affiliation: | 1. School of Chemistry and Chemical Engineering, State Key Laboratory of Metal Matrix Composites, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai, 200240 China Key Laboratory of Flexible Electronics (KLOFE) &, Institute of Advanced Materials (IAM), Jiangsu National Synergetic Innovation Center for Advanced, Materials (SICAM), Nanjing Tech University (NJTECH), 30 South Puzhu Road, Nanjing, 211816 China These authors contributed equally to this work.;2. Departments of Radiology and Cardiology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai Jiao Tong University School of Medicine, 600 Yishan Road, Shanghai, 200233 China These authors contributed equally to this work.;3. Key Laboratory of Flexible Electronics (KLOFE) &, Institute of Advanced Materials (IAM), Jiangsu National Synergetic Innovation Center for Advanced, Materials (SICAM), Nanjing Tech University (NJTECH), 30 South Puzhu Road, Nanjing, 211816 China;4. School of Chemistry and Chemical Engineering, State Key Laboratory of Metal Matrix Composites, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai, 200240 China;5. Departments of Radiology and Cardiology, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai Jiao Tong University School of Medicine, 600 Yishan Road, Shanghai, 200233 China |
| Abstract: | DNA-modified lanthanide-doped upconversion nanoparticles (DNA-UCNPs) that combine the functions of DNA and the optical features of UCNPs have shown great promise in a wide range of fields. However, challenges remain in precisely tethering and orienting the DNA strands on the UCNP surface. Herein, we systematically investigate the sequence dependence of DNAs in their interactions with UCNPs, and reveal that poly-cytosine (poly-C) has high affinity for the UCNP surface. A general approach to synthesize monodispersed DNA-UCNP conjugates is developed using poly-C-containing diblock DNA strands. The poly-C segment of the DNA strand binds to the surfaces of UCNPs and the second segment is oriented perpendicularly on the UCNP surface, making the DNA-UCNPs highly stable and monodispersed in aqueous solution. The dense layer of DNA on the UCNP surface enables the programmable assembly of UCNPs with other DNA-functionalized nanoparticles or DNA origamis through hybridization, resulting in the formation of well-organized complex structures. |
| Keywords: | DNA Nanostrukturen Polycytosin Upconversion-Nanopartikel Selbstassemblierung |