| Institution: | 1. Department of Food Science & Engineering, Ewha Womans University, Seoul, 03760 Republic of Korea;2. Department of Food Science & Engineering, Ewha Womans University, Seoul, 03760 Republic of Korea
Department of Chemistry and Nanoscience, Ewha Womans University, Seoul, 03760 Republic of Korea;3. Department of Chemistry and Nanoscience, Ewha Womans University, Seoul, 03760 Republic of Korea;4. Institute of Biochemistry, Department of Biotechnology & Enzyme Catalysis, Greifswald University, 17487 Greifswald, Germany;5. Department of Biotechnology, Delft University of Technology, Van der Maasweg 9, 2629HZ Delft, The Netherlands;6. Department of Bioscience and Biotechnology, Konkuk University, Seoul, 05029 Republic of Korea |
| Abstract: | Long-chain aliphatic amines such as (S,Z)-heptadec-9-en-7-amine and 9-aminoheptadecane were synthesized from ricinoleic acid and oleic acid, respectively, by whole-cell cascade reactions using the combination of an alcohol dehydrogenase (ADH) from Micrococcus luteus, an engineered amine transaminase from Vibrio fluvialis (Vf-ATA), and a photoactivated decarboxylase from Chlorella variabilis NC64A (Cv-FAP) in a one-pot process. In addition, long chain aliphatic esters such as 10-(heptanoyloxy)dec-8-ene and octylnonanoate were prepared from ricinoleic acid and oleic acid, respectively, by using the combination of the ADH, a Baeyer–Villiger monooxygenase variant from Pseudomonas putida KT2440, and the Cv-FAP. The target compounds were produced at rates of up to 37 U g?1 dry cells with conversions up to 90 %. Therefore, this study contributes to the preparation of industrially relevant long-chain aliphatic chiral amines and esters from renewable fatty acid resources. |
