Characterization of [2Fe–2S]-Cluster-Bridged Protein Complexes and Reaction Intermediates by use of Native Mass Spectrometric Methods |
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Authors: | Mengxuan Jia Sambuddha Sen Christine Wachnowsky Insiya Fidai Prof James A Cowan Prof Vicki H Wysocki |
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Institution: | Department of Chemistry and Biochemistry, The Ohio State University, Columbus, OH, 43210 USA |
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Abstract: | Many iron–sulfur proteins involved in cluster trafficking form 2Fe–2S]-cluster-bridged complexes that are often challenging to characterize because of the inherent instability of the cluster at the interface. Herein, we illustrate the use of fast, online buffer exchange coupled to a native mass spectrometry (OBE nMS) method to characterize 2Fe–2S]-cluster-bridged proteins and their transient cluster-transfer intermediates. The use of this mechanistic and protein-characterization tool is demonstrated with holo glutaredoxin 5 (GLRX5) homodimer and holo GLRX5:BolA-like protein 3 (BOLA3) heterodimer. Using the OBE nMS method, cluster-transfer reactions between the holo-dimers and apo-ferredoxin (FDX2) are monitored, and intermediate 2Fe–2S] species, such as (FDX2:GLRX5:2Fe–2S]:GSH) and (FDX2:BOLA3:GLRX5:2Fe–2S]:GSH) are detected. The OBE nMS method is a robust technique for characterizing iron–sulfur-cluster-bridged protein complexes and transient iron–sulfur-cluster transfer intermediates. |
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Keywords: | Cofaktoren Eisen-Schwefel-Cluster Native Massenspektrometrie Proteinkomplexe Strukturbiologie |
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