Harnessing Effective Molarity to Design an Electrochemical DNA-based Platform for Clinically Relevant Antibody Detection |
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Authors: | Dr Marianna Rossetti Simone Brannetti Dr Marco Mocenigo Dr Bruna Marini Dr Rudy Ippodrino Prof Alessandro Porchetta |
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Institution: | 1. Department of Chemistry, University of Rome Tor Vergata, Via della Ricerca Scientifica, 00133 Rome, Italy;2. Ulisse BioMed S.r.l., Area Science Park, 34149 Trieste, Italy |
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Abstract: | Easy-to-use platforms for rapid antibody detection are likely to improve molecular diagnostics and immunotherapy monitoring. However, current technologies require multi-step, time-consuming procedures that limit their applicability in these fields. Herein, we demonstrate effective molarity-driven electrochemical DNA-based detection of target antibodies. We show a highly selective, signal-on DNA-based sensor that takes advantage of antibody-binding-induced increase of local concentration to detect clinically relevant antibodies in blood serum. The sensing platform is modular, rapid, and versatile and allows the detection of both IgG and IgE antibodies. We also demonstrate the possible use of this strategy for the monitoring of therapeutic monoclonal antibodies in body fluids. Our approach highlights the potential of harnessing effective molarity for the design of electrochemical sensing strategies. |
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Keywords: | antibody monitoring biosensors DNA nanotechnology effective molarity electrochemistry |
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