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Machine-Driven Chemoenzymatic Synthesis of Glycopeptide |
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| Authors: | Jiabin Zhang Ding Liu Varma Saikam Madhusudhan R. Gadi Christopher Gibbons Xuan Fu Heliang Song Jin Yu Shukkoor M. Kondengaden Prof. Dr. Peng G. Wang Prof. Dr. Liuqing Wen |
| Affiliation: | 1. Department of Chemistry, Georgia State University, Atlanta, GA, 30303 USA These authors contributed equally to this work.;2. Department of Chemistry, Georgia State University, Atlanta, GA, 30303 USA;3. Imperial College London, Du Cane Road, London, W12 0NN UK;4. School of Medicine, Southern University of Science and Technology, Shenzhen, China |
| Abstract: | Historically, researchers have put considerable effort into developing automation systems to prepare natural biopolymers such as peptides and oligonucleotides. The availability of such mature systems has significantly advanced the development of natural science. Over the past twenty years, breakthroughs in automated synthesis of oligosaccharides have also been achieved. A machine-driven platform for glycopeptide synthesis by a reconstructed peptide synthesizer is described. The designed platform is based on the use of an amine-functionalized silica resin to facilitate the chemical synthesis of peptides in organic solvent as well as the enzymatic synthesis of glycan epitopes in the aqueous phase in a single reaction vessel. Both syntheses were performed by a peptide synthesizer in a semiautomated manner. |
| Keywords: | automation chemoenzymatic synthesis machine-driven peptide synthesizer silica resin |