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Optimization of Flavonoid Extraction from Xanthoceras sorbifolia Bunge Flowers,and the Antioxidant and Antibacterial Capacity of the Extract
Authors:Henghui Zhang  Xiaoli Wang  Dongliang He  Dongliang Zou  Runzhu Zhao  Huifang Wang  Shuying Li  Yongping Xu  Buhailiqiemu Abudureheman
Affiliation:1.Department of Environment and Safety Engineering, Taiyuan Institute of Technology, Taiyuan 030008, China; (H.Z.); (X.W.); (D.H.); (D.Z.); (R.Z.); (H.W.);2.SEM Bio-Engineering Technology Co., Ltd., Dalian 116600, China;3.School of Bioengineering, Dalian University of Technology, Dalian 116024, China;4.School of Food Science and Engineering, Xinjiang Institute of Technology, Aksu 843100, China
Abstract:In the present work, the extraction process of total flavonoids (TFs) from X. sorbifolia flowers by ultrasound-assisted extraction was optimized under the response surface methodology (RSM) on the basis of single-factor experiments. The optimal extraction conditions were as follows: ethanol concentration of 80%, solid–liquid ratio of 1:37 (g/mL), temperature of 84 °C, and extraction time of 1 h. Under the optimized conditions, the extraction yield of the TFs was 3.956 ± 0.04%. The radical scavenging capacities of TFs against 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) were much greater than that of rutin. The results of antibacterial experiments indicated that the TFs displayed strong inhibitory activities on E. coli, S. aureus and Bacillus subtilis. Therefore, X. sorbifolia flowers can be used as a novel source of natural flavonoids, and the TFs have potential applications as natural antioxidants or antibacterial agents in the food and pharmaceutical industries.
Keywords:Xanthoceras sorbifolia Bunge   total flavonoids   ultrasound-assisted extraction   response surface methodology   antioxidant activity   antibacterial activity
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