An Electrochemical Immunosensor Based on SPA and rGO-PEI-Ag-Nf for the Detection of Arsanilic Acid |
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| Authors: | Yanwei Wang Dongdong Ma Gaiping Zhang Xuannian Wang Jingming Zhou Yumei Chen Xiaojuan You Chao Liang Yanhua Qi Yuya Li Aiping Wang |
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| Affiliation: | 1.School of Life Science, Zhengzhou University, Zhengzhou 450000, China; (Y.W.); (D.M.); (G.Z.); (J.Z.); (Y.C.); (X.Y.); (C.L.); (Y.Q.); (Y.L.);2.School of Life Science and Basic Medicine, Xinxiang University, Xinxiang 453003, China; |
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| Abstract: | A sensitive electrochemical immunosensor was prepared for rapid detection of ASA based on arsanilic acid (ASA) monoclonal antibody with high affinity. In the preparation of nanomaterials, polyethyleneimine (PEI) improved the stability of the solution and acted as a reducing agent to generate reduced graphene oxide (rGO) with relatively strong conductivity, thereby promoting the transfer of electrons. The dual conductivity of rGO and silver nanoparticles (AgNPs) improved the sensitivity of the sensor. The synthesis of nanomaterials were confirmed by UV-Vis spectroscopy, X-ray diffraction, transmission electron microscopy and scanning electron microscopy. In the optimal experiment conditions, the sensor could achieve the detection range of 0.50–500 ng mL−1 and the limit of detection (LOD) of 0.38 ng mL−1 (S/N = 3). Moreover, the sensor exhibited excellent specificity and acceptable stability, suggesting that the proposed sensor possessed a good potential in ASA detection. Thus, the as-prepared biosensor may be a potential way for detecting other antibiotics in meat and animal-derived foods. |
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| Keywords: | arsanilic acid polyethyleneimine silver nanoparticles reduced graphene oxide staphylococcal protein A electrochemical immunosensor |
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