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Flow injection analysis : Part VI. The determination of phosphate and chloride in blood serum by dialysis and sample dilution
Authors:Elo H. Hansen  Jaromir Růžička
Affiliation:Chemistry Department A, The Technical University of Denmark, Building 207, DK-2800 LyngbyDenmark
Abstract:The rapid determination of chloride and inorganic phosphate in blood serum based on continuous flow spectrophotometry was adapted to Flow Injection Analysis by applying dialysis as well as differential dilution of the injected samples. In addition to the manual injection procedure, an automated microsampling unit was developed and used. By continuous dialysis, chloride could be determined reproducibly at a sampling rate of 125 samples per hour at sample volumes of 60 μl or less. A similar sampling rate and high precision were obtained for the determination of inorganic phosphate by using differential dilution.
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