Untersuchungen zur aufklärung des mechanismus des redoxsystems flavinmononucleotid/dihydroflavinmononucleotid: III. Die abhängigkeit des gleichgewichtspotentials vom reduktionsgrad der FMN-Lösung

A comparison was made between the experimental and the calculated dependence of the equilibrium potential on the ratio of the diffusion currents (E ? log(i_K,k/i_D,a)) for various combinations of intermediates. In addition the observed equilibrium potential was correlated to the calculated ratio log(FMN]/FMNH₂]). A good correlation is obtained if the following intermediates are assumed to occur on FMN reduction in 0.05 M H₂SO₄: FMNH^., (FMN·FMNH₂) and (FMNH^.·FMNH₂); it must also be assumed that the last named intermediate is non-reducible.