High-performance liquid chromatographic assay for amiodarone N-deethylation in microsomes of rat liver. |
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Authors: | J M Trivier J Pommery C Libersa J Caron M Lhermitte |
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Institution: | Département de Biochimie, H?pital Calmette, Lille, France. |
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Abstract: | A reversed-phase high-performance liquid chromatographic assay using ultraviolet detection is described for determining the production of the major N-dealkylated metabolite of amiodarone in rat liver microsomes. The principal advantages of this method are its simple sample preparation (protein precipitation by acetonitrile), low detection limit for N-desethylamiodarone (0.05 mumol/l) and relatively short analysis time (16 min). Its analytical applicability is demonstrated by the comparison of the kinetic parameters (maximum velocity and Michaelis-Menten constant) between Sprague-Dawley and Dark-Agouti rats. |
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