|
|||||
|
|
Production of thermostable multiple enzymes from Bacillus amyloliquefaciens KUB29 |
|
| Authors: | Kubendran Devaraj Salma Aathika Karthik Periyasamy Premkumar Manickam Periyaraman Shanmugam Palaniyandi |
| Institution: | 1. Environmental Management Lab, Department of Applied Science &2. Technology, A.C. Tech, Anna University, Chennai, India;3. Environmental Technology Division, Central Leather Research Institute, Chennai, India |
| Abstract: | A strain of Bacillus amyloliquefaciens KUB29 was identified by 16S ribosomal RNA sequencing (Genbank: MF772779.1). Production of thermostable protease, amylase and lipase were done by the isolated strain. The produced enzymes were partially purified by ammonium precipitation followed by dialysis process. Protease and lipase enzymes are effectively used in bio-oil extraction from proteinaceous sample followed by transesterification to produce methyl ester. Amylase enzyme is widely used in food and laundry industry. The produced enzymes are active at thermophilic condition of 55 °C. Use of these enzymes in biofuel production process will make the process cleaner and greener. |
| Keywords: | Isolation thermostable enzyme assay biofuel ammonium precipitation |