Dereplication and targeted isolation of bioactive sulphur compound from bacteria isolated from a hydrothermal field |
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| Authors: | Xiuping Lin Kunlong Li Lilin Yang Xiaoying Peng Wei Fang |
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| Institution: | 1. Chinese Academy of Sciences (CAS) Key Laboratory of Tropical Marine Bio-resources and Ecology/Guangdong Key Laboratory of Marine Materia Medica/RNAM Center for Marine Microbiology, South China Sea Institute of Oceanology, CAS, Guangzhou, China;2. College of Earth Sciences, University of Chinese Academy of Sciences, Beijing, China;3. Hubei Biopesticide Engineering Research Center, Hubei Academy of Agricultural Science, Wuhan, China |
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| Abstract: | Marine micro-organisms in the deep-sea hydrothermal vent systems are considered as potential sources of bioactive natural products. Sixteen bacterial strains were isolated from a deep-sea hydrothermal field and screened for bioactive metabolism studies. After the strains were subjected to bioactive testing at different culture media, chemical dereplication by HPLC coupled to high-resolution mass spectrometer was performed to analyse or determine the main secondary metabolisms in those strains. Strain 06204 was large-scale fermented with relative optimal media, for isolating the desired sulphur compound. Butyrolactone I 3-sulphate was isolated and structurally identified from the extract, guided by dereplication and showed moderate antivirus activities against H3N2 and EV71 viruses. Our study suggests that deep-sea hydrothermal bacteria are good sources of sulphur natural products. Meanwhile, the described approach, mainly bioactive screening, dereplication and targeted isolation, is effective and efficient to discover interesting bioactive compounds in hydrothermal bacteria. |
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| Keywords: | Hydrothermal bacteria dereplication targeted isolation antivirus butyrolactone I 3-sulphate |
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