Pradimicin-IRD from Amycolatopsis sp. IRD-009 and its antimicrobial and cytotoxic activities |
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| Authors: | Anelize Bauermeister Felipe A Calil Francisco das C L Pinto Talita C T Medeiros Larissa C Almeida Leonardo J Silva |
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| Institution: | 1. Departamento de Química, Faculdade de Filosofia, Ciências e Letras de Ribeir?o Preto, Universidade de S?o Paulo, Ribeir?o Preto, Brazil;2. Departamento de Química Organica e Inorganica, Universidade Federal do Ceará, Fortaleza, Brazil;3. Departamento de Farmacologia, Instituto de Ciências Biomédicas, Universidade de S?o Paulo, S?o Paulo, Brazil;4. College of Agriculture “Luiz de Queiroz”, Universidade de S?o Paulo, Piracicaba, Brazil |
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| Abstract: | A new polycyclic antibiotic, pradimicin-IRD, was isolated from actinobacteria Amycolatopsis sp. IRD-009 recovered from soil of Brazilian rainforest undergoing restoration area. This molecule is the major compound produced in solid culture media. The new compound was detected by a focused method of precursor ion (high-performance liquid chromatography coupled to tandem mass spectrometer) developed previously to identify unusual aminoglycosyl sugar moieties. The compound was isolated and its structure was, therefore, elucidated by high-resolution mass spectrometry, and 1D and 2D nuclear magnetic resonance experiments. Pradimicin-IRD displayed potential antimicrobial activity against Streptococcus agalactiae (MIC 3.1 μg/mL), Pseudomonas aeruginosa (MIC 3.1 μg/mL) and Staphylococcus aureus (MIC 3.1 μg/mL), and also cytotoxicity against tumour and non-tumour cell lines with IC50 values ranging from 0.8 μM in HCT-116 colon carcinoma cells to 2.7 μM in MM 200 melanoma cells. Particularly, these biological properties are described for the first time for this chemical class. |
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| Keywords: | Actinobacteria antibacterial activity cytotoxic activity natural products |
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