Chemical constituents of Oldenlandia pinifolia and their antiproliferative activities |
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| Authors: | Nguyen Thi Hoang Anh Khieu Thi Tam Nguyen Van Tuan Dao Duc Thien Tran Duc Quan Nguyen Thanh Tam |
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| Institution: | 1. Institute of Chemistry, Vietnam Academy of Science and Technology , Hanoi, Vietnam;2. Graduate University of Science and Technology, Vietnam Academy of Science and Technology , Hanoi, Vietnam hoanganhvhh@gmail.com nthanh@ich.vast.vn;3. Graduate University of Science and Technology, Vietnam Academy of Science and Technology , Hanoi, Vietnam;4. Thai Nguyen University of Sciences, Thai Nguyen University , Thai Nguyen, Vietnam;5. Asean College , Van Lam, Hung Yen, Vietnam;6. Institute of Chemistry, Vietnam Academy of Science and Technology , Hanoi, Vietnam |
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| Abstract: | This study describes the chemical constituents of Oldenlandia pinifolia (Wall. Ex G. Don) Kuntze (synonym Hedyotis pinifolia Wall. Ex G. Don) and discusses their anti-proliferative activities. Thirteen compounds were isolated from the n-hexane, ethyl acetate and n-butanol extracts of whole plants O. pinifolia by chromatography method. Their structures were elucidated using MS and NMR analysis and compared with reported data. They are three anthraquinones, a carotenoid, two triterpenes, four iridoid glycosides and three flavonoid glycosides. Among them, 2-methyl-1,4,6-trihydroxy-anthraquinone is a new one, and three compounds were found for the first time in this genus. MTT assay resulted that the n-butanol extract and four isolated compounds inhibited the proliferation of chronic myelogenous leukaemia cells. The results from Hoechst 33343 staining and caspase 3-inducing exhibited that those four tested compounds induced apoptosis and activated caspase 3 (p < 0.05). One of them, isorhamnetin-3-O-β-rutinoside showed the most activity with IC50 value of 394.68 ± 25.12 μM. |
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| Keywords: | Oldenlandia pinifolia (Hedyotis pinifolia) anti-proliferation activity caspase 3 apoptosis |
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