Chemical characterisation and hepatoprotective potential of Cosmos sulphureus Cav. and Cosmos bipinnatus Cav. |
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| Authors: | Mohammad Saleem Hafiz Akbar Ali Muhammad Furqan Akhtar Uzma Saleem Ammara Saleem Iram Irshad |
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| Institution: | 1. Faculty of Pharmaceutical Sciences, Department of Pharmacology, GC University Faisalabad , Faisalabad, Pakistan msaleem0141@hotmail.com saleem2978@hotmail.com;2. Faculty of Pharmaceutical Sciences, Department of Pharmacology, GC University Faisalabad , Faisalabad, Pakistan |
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| Abstract: | This study was conducted to validate the hepatoprotective activity of Cosmos sulphureus and Cosmos bipinnatus. Aqua-methanolic extracts of both plants were evaluated for the presence of various phyto-constituents through HPLC. Different doses of both plant extracts were administered to rats for nine days. Standard control was silymarin 100 mg/kg. Paracetamol 1 gm/kg was administered 3 h post treatment on 9th day for induction of hepatotoxicity. Blood was collected for the evaluation of liver biochemical markers and livers were removed for histopathological evaluation 24 h post-paracetamol treatment. HPLC analysis revealed the presence of quercetin, gallic acid, caffeic acid and chlorogenic acid in both plant extracts. The extracts of both plants decreased the level of alanine aminotransaminase and total bilirubin significantly (p < 0.05), dose dependently and protected hepatocytes from paracetamol-induced hepatotoxicity. It can be concluded that both plants may possess hepatoprotective activity possibly due to the presence of quercetin and phenolic compounds. |
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| Keywords: | Hepatoprotective paracetamol HPLC quercetin gallic acid |
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