Incorporation of Baccharis dracunculifolia DC (Asteraceae) leaf extract into phosphatidylcholine-cholesterol liposomes improves its anti-inflammatory effect in vivo |
| |
| Authors: | Andréa Silva Garcia de Figueiredo-Rinhel Micássio Fernandes de Andrade Ana Paula Landi-Librandi Ana Elisa Caleiro Seixas Azzolini Luciana Mariko Kabeya Jairo Kenupp Bastos |
| |
| Institution: | 1. Departamento de Física e Química, Faculdade de Ciências Farmacêuticas de Ribeir?o Preto da Universidade de S?o Paulo, Ribeir?o Preto, Brazil;2. Departamento de Bioquímica e Imunologia, Faculdade de Medicina de Ribeir?o Preto da Universidade de S?o Paulo, Ribeir?o Preto, Brazil;3. Departamento de Ciências Farmacêuticas, Faculdade de Ciências Farmacêuticas de Ribeir?o Preto da Universidade de S?o Paulo, Ribeir?o Preto, Brazil |
| |
| Abstract: | The aerial parts of Baccharis dracunculifolia (BdE) is used in the Brazilian folk medicine to treat inflammatory conditions. Here we examined the ability of free and liposomal BdE to modulate reactive oxygen species generation in human neutrophils in vitro and zymosan-induced acute joint inflammation in Wistar rats. We prepared biocompatible liposomes of soya phosphatidylcholine and cholesterol with low diameter, homogeneous size distribution, and neutral surface charge. Free BdE decreased joint swelling, total leucocyte and neutrophil infiltration, and the synovial levels of tumour necrosis factor-α and interleukins 6 and 1β. Incorporation of BdE into liposomes preserved its capacity to inhibit the neutrophil superoxide anion and total reactive oxygen species generation, and improved its anti-inflammatory effect in vivo by decreasing the effective BdE dose by nearly sixfold. The same liposome type lowered the effective dose of caffeic acid by nearly sixteenfold. Therefore, incorporation of BdE into phosphatidylcholine-cholesterol liposomes improves its anti-inflammatory effect. |
| |
| Keywords: | Baccharis dracunculifolia caffeic acid neutrophil anti-inflammatory liposome arthritis |
|
|
