Anti-hydroxyl radical activity,redox potential and anti-AChE activity of Amanita strobiliformis polysaccharide extract |
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| Authors: | Maja Karaman Ljiljana Janjušević Dragica Jakovljević Filip Šibul |
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| Affiliation: | 1. Faculty of Sciences, Department of Biology and Ecology, University of Novi Sad, Novi Sad, Serbia;2. Institute of Chemistry, Technology and Metallurgy, Centre of Chemistry, University of Belgrade, Belgrade, Serbia;3. Faculty of Sciences, Department of Chemistry, Biochemistry and Environmental Protection, University of Novi Sad, Novi Sad, Serbia |
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| Abstract: | This study outlines antioxidant and anti-AChE activities of the polysaccharide (PSH) extract from the mushroom species Amanita strobiliformis. Both the presence of α and ß glucans within the aforementioned extract was recorded. PSH extract displayed a profound scavenging activity of OH radicals (IC50 value, 11.86 ± 0.59 μg/mL) and high potential for reduction of Fe3+ ions (174.11 ± 8.70 mg eq. AA/g d.w.) being almost 48- and 5-fold more effective than mannitol and butylated hydroxytoluene used as a positive control, respectively. Compared with galanthamine (0.001 μg), the same extract exhibited a moderate anti-AChE activity (10 μg) in solid. Since purified PSH extract exhibited higher bioactivity (IC50 value 7.27 ± 0.31 μg/mL, 197.68 ± 9.47 mg eq. AA/g d.w. and 0.1 μg, respectively), it can be predominantly ascribed to the polysaccharide compounds. A. strobiliformis PSH extract may be considered as a promising resource of potent bioactive polysaccharides of natural origin successfully addressing both oxidative stress and lack of acetylcholine. |
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| Keywords: | Mushrooms Amanitaceae polar extract biological activity |
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