Applications of synthetic oligoribonucleotide analogues in studies of RNA structure and function |
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Authors: | Jane A Grasby Clare E Pritchard and Michael J Gait |
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Institution: | (1) MRC Laboratory of Molecular Biology, Hills Road, CB2 2QH Cambridge, UK;(2) Present address: Department of Biochemistry, University of Oxford, OX1 3QU Oxford |
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Abstract: | The study of RNA structure and function has been considerably aided by the development of methods for the chemical synthesis
of oligoribonucleotides into which have been incorporated modified nucleosides carrying site-specific alterations. Such modifications
are designed to eliminate or alter individual functional groups in the RNA which potentially can take part in hydrogen-bonding
or other non-covalent interactions. Comparison of the properties of the modified RNA with unmodified RNA models allows conclusions
to be drawn concerning the importance or otherwise of specific functional groups within the RNA. The methods have been applied
to studies of RNA structure, RNA catalysis, and interactions of RNA with proteins. |
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Keywords: | Oligoribonucleotides modified nucleosides ribozyme protein-RNA recognition |
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