Preparation and electron microscopy of liquid crystalline condensates of DNA |
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| Authors: | B. Samorì I. Domini Pellerano A. Rossi D. Grasso G. Albertini |
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| Affiliation: | (1) Dipartimento di Chimica Organica e di Chimica Industriale e dei Materiali, Università di Bologna, Via Risorgimento 4, 40136 Bologna, Italia;(2) Dipartimento di Chimica dell’Università, Catania, Italia;(3) Dipartimento di Scienze dei Materiali e della Terra, Università di Ancona, Ancona, Italia |
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| Abstract: | Summary A brief survey of thein vitro methods of preparing DNA mesomorphic phases is presented. The significance of thesein vitro preparations for thein vivo condensed states of DNA is also discussed. The procedure of preparing a dispersion of mesomorphic condensates of unsonicated calf thymus DNA by using polyethylene glycol was investigated by the circular dichroism (CD) technique. The operational conditions able to provide at the same time good packing of the DNA chains and reproducible CD signals were searched. The morphology of the condensates thus obtained was studied by transmission electron microscopy. Work presented at the second USSR-Italy Bilateral Meeting on Liquid Crystals held in Moscow, September 15–21, 1988. |
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| Keywords: | Liquid crystals |
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