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| 大鼠尿液中异丙嗪及其主要代谢物的GC-MS分析 | |
| 引用本文： | 黄克建,朱定姬,李宏森,林翠梧,李璐,刘晓锋.大鼠尿液中异丙嗪及其主要代谢物的GC-MS分析[J].分析测试学报,2010,29(3). |
| 作者姓名： | 黄克建 朱定姬 李宏森 林翠梧 李璐 刘晓锋 |
| 作者单位： | 1. 广西壮族自治区公安厅物证鉴定中心,广西,南宁,530012 2. 广西大学,化学化工学院,广西,南宁,530004 3. 广西壮族自治区公安厅物证鉴定中心,广西,南宁,530012;广西大学,化学化工学院,广西,南宁,530004 |
| 基金项目： | 公安部应用创新计划资助项目，广西壮族自治区公安厅科学研究与技术开发计划资助项目(桂公 |
| 摘 要： | 采用气相色谱-质谱(GC-MS)分析了大鼠尿液中的异丙嗪及其代谢产物。尿液经β-葡萄糖醛酸酶酶解,Oasis(HLB 3 mL固相萃取柱提取后,以Restek R tx-5MS(30 m×0.25 mm×0.25μm)石英毛细管色谱柱为分析柱,经GC-MS分离检测。根据质谱图对代谢物的结构进行鉴定。结果表明:在大鼠尿液中检出了吩噻嗪、去甲基异丙嗪、异丙嗪亚砜、2-羟基去甲基异丙嗪、二羟基异丙嗪、顺-N-(1-丙烯基)吩噻嗪、反-N-(1-丙烯基)吩噻嗪、顺-N-(1-丙烯基)吩噻嗪砜、反-N-(1-丙烯基)吩噻嗪砜、顺-N-(1-丙烯基)吩噻嗪亚砜及反-N-(1-丙烯基)吩噻嗪亚砜11种代谢物。对异丙嗪在大鼠体内的氧化、还原等主要代谢途径进行了讨论。 |
| 关 键 词： | 异丙嗪 尿液 代谢物 气相色谱-质谱 |
Analysis of Promethazine and Its Metabolites in Rats Urine by Gas Chromatography-Mass Spectrometry |
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| HUANG Ke-jian,ZHU Ding-ji,LI Hong-sen,LIN Cui-wu,LI Lu,LIU Xiao-feng.Analysis of Promethazine and Its Metabolites in Rats Urine by Gas Chromatography-Mass Spectrometry[J].Journal of Instrumental Analysis,2010,29(3). | |
| Authors: | HUANG Ke-jian ZHU Ding-ji LI Hong-sen LIN Cui-wu LI Lu LIU Xiao-feng |
| Institution: | HUANG Ke-jian1,ZHU Ding-ji2,LI Hong-sen1,LIN Cui-wu2,LI Lu1,LIU Xiao-feng1,2(1.Institute of Forensic Science,Public Security Department of Guangxi Zhuang Autonomous Region,Nanning 530012,China,2.School of Chemistry , Chemical Engineering,Guangxi University,Nanning 530004,China) |
| Abstract: | A gas chromatography and mass spectrometric(GC-MS) method was developed for the analysis of promethazine(PMZ) and its metabolites in wistar rat with the electron ionization(EI) and the positive chemical ionization(PCI) mode.The sample was hydrolyzed with β-glucuronidase,and extracted by a Oasis~((R)) HLB 3 mL solid phase extraction(SPE).The separation of target compounds was performed on a Restek Rtx-5 MS(30 m×0.25 mm×0.25 μm) capillary column.The molecular structures of the metabolites were identified based on their MS spectra.The result indicated that PMZ and its eleven metabolites,including phenothiazine,desmethylpromethazine,promethazine sulphoxide,2-hydroxy-desmethylpromethazine,dihydroxypromethazine,cis-N-(1-propenyl) phenothiazine,trans-N-(1-propenyl) phenothiazine,cis-N-(1-propenyl) phenothiazine-5-oxide,trans-N-(1-propenyl) phenothiazine-5-oxide,cis-N-(1-propenyl) phenothiazine-5,5-dioxide,trans-N-(1-propenyl) phenothiazine-5,5-dioxide were found in rat urine.The main metabolic process of PMZ in rat bio-body was also discussed. |
| Keywords: | promethazine urine metabolites GC-MS |
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