STUDIES ON THE PROPERTY OF SULFHYDRYL BINDING SITE ON THE LUNG NORMAL AND CANCER CELL MEMBRANE OF CHINESE HAMSTER WITH MALEIMIDE SPIN LABELS |
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作者姓名: | 忻文娟 赵保路 张建中 |
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作者单位: | Institute of Biophysics,Academia Sinica,Beijing,Institute of Biophysics,Academia Sinica,Beijing,Institute of Biophysics,Academia Sinica,Beijing |
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摘 要: | In this paper, five maleimide spin labels with different chain lengths were used to studythe properties of binding sites of sulfhydryl groups on the meanbrane proteins of normal cellV_79 and cancer cells V_79-B_1 in the lung of Chinese hamster. The ratio of the strongly immobi-lized component to the weakly immobilized eomponent (s/w) and the rotational correlation time(τ_e) were calculated on the basis of ESR spectra. From the varying of the s/w and τ_c withthe ehain lengths of spilt labels it was deducted that the binding sites of sulfhydrylgroups on V_79 and V_79-B_1 membrane proteins were of eonical shape and that the binding sitesof sulfhydryl groups on V_79 membrane protein were deeper and narrower than those on V_79-B_1.
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