1. Chemical Biology Laboratory, National Cancer Institute, 376 Boyles Street, Frederick, MD 21702, USA;2. Department of Chemistry and Biochemistry, University of Delaware, Newark, DE 19716, USA
Abstract:
Download : Download high-res image (111KB)
Download : Download full-size image
Highlights? Capillary electrophoresis assay using a mobility shift as a readout to study sumoylation ? Assay identified a 2-D08 (2′,3′,4′-trihydroxyflavone) as a potent sumoylation inhibitor ? 2-D08 acts by inhibiting the transfer of SUMO-1 from the E2 thioester to the SUMO substrate ? 2-D08 inhibits sumoylation of topo-I in two different breast cancer cell lines
