基于聚对苯二甲酸乙二醇酯薄膜的石墨烯/金纳米粒子/葡萄糖氧化酶柔性电极的制备及检测葡萄糖

采用化学气相沉积法生长多晶石墨烯(Graphene, G),转移至聚对苯二甲酸乙二醇酯(PET)薄膜表面,通过控制金溶胶蒸发速率,在多晶石墨烯表面组装均匀分布的亚单层金纳米粒子(AuNPs);然后修饰巯基乙酸,通过共价交联反应将葡萄糖氧化酶固定于AuNPs表面,构建基于PET膜的石墨烯/金纳米粒子/葡萄糖氧化酶(G/AuNPs/GOD)柔性电极.此电极在工作电位0.6 V(vs.SCE电极)、pH 7.0磷酸盐缓冲溶液、室温25℃条件下,差分脉冲伏安法响应电流与被测葡萄糖浓度在0.05~10.55 mmol/L范围内呈线性关系,线性方程为I(108A)=0.2629 C(mmol/L)+1.4149,线性相关系数 r=0.9955,检出限1 μmol/L (3σ). G/AuNPs/GOD柔性电极的制备可为特定环境和可穿戴设备的葡萄糖检测提供了新的途径和方法,拓展了葡萄糖检测的应用范围.

Preparation of Graphene/Gold Nanoparticles/Glucose Oxidase Flexible Electrode Based on Poly(ethylene terephthalate) Film and Its Application in Detection of Glucose

The graphene prepared by chemical vapor deposition onto copper foils was transferred onto a poly(ethylene terephthalate) (PET) soft substrate by the aid of poly-methyl methacrylate (PMMA).The soft G/AuNPs/GOD composite electrode based on PET substrate was fabricated using a protocol in which the uniform distribution of Au nanoparticles (AuNPs) was firstly obtained by controlling the evaporation of gold sol on a graphene surface, then thioglycolic acid (TGA) was modified on the AuNPs through Au-S bond, and finally glucose oxidase (GOD) was immobilized on the surface of AuNPs through acylation reaction between TGA and the GOD.Glucose was detected in the linear range from 0.05 to 10.55 mmol/L with a linear correlation coefficient (r) of 0.9955.The detection was performed in phosphate buffer solution (pH 7) at 25℃ with a working potential of 0.6 V (vs.SCE electrode), and the detection limit was 1 μmol/L (3σ).The G/AuNPs/GOD flexible electrode based on PET substrate provided a new pathway to detect glucose in special environments using wearable equipment, which enlarged the applied field of glucose detection.