超声萃取-高效液相色谱法同时测定人造革中7种苯并三唑类紫外线吸收剂含量

建立了超声萃取-高效液相色谱法(HPLC)同时测定人造革中7种苯并三唑类紫外线吸收剂UV-P、UV-326、UV-327、UV-329、UV-350、UV-320和UV-328含量的方法。该方法以甲醇为萃取溶剂,超声萃取人造革中的苯并三唑类紫外线吸收剂,萃取液经处理后直接进行HPLC分析,外标法定量。在信噪比(S/N)=3的条件下,UV-P、UV-326、UV-327、UV-329、UV-350的检出限均为0.05mg/kg,UV-320、UV-328的检出限均为0.10 mg/kg。在3个不同加标浓度水平下,各组分的平均加标回收率为88.19%~98.32%,相对标准偏差(RSD)为0.61%~3.74%。该方法简便快捷、灵敏度高,可用于人造革中苯并三唑类紫外线吸收剂的测定。采用该方法对市售人造革样品进行测定,结果在6个样品中检出了不同浓度水平的UV-P和UV-329。

Simultaneous Determination of Seven Benzotriazole Ultraviolet Absorbers in Artificial Leathers by High-performance Liquid Chromatography Coupled with Ultrasonic Extraction

A high-performance liquid chromatographic method was established for the simultaneous determination of seven benzotriazole ultraviolet absorbers in artificial leathers.Benzotriazole ultraviolet absorbers in artificial leathers were ultrasonically extracted using methanol as the extraction solvent.The extract was then analyzed by high-performance liquid chromatography (HPLC) technique.The concentration of each analyte was calibrated by external standard method.At the condition of signal to noise(S/N) of three,the limits of detection were 0.05 mg/kg for UV-P,UV-326,UV-327,UV-329 and UV-350,0.10 mg/kg for UV-320 and UV-328,respectively.The recoveries changed from 88.19％ to 98.32％ under three different spiked levels while the relative standard deviation varied from 0.61％ to 3.74％.The proposed method was simple,rapid,sensitive,and applicable to the determination of benzotriazole ultraviolet absorbers in artificial leathers.The proposed method was applied in the determination of benzotriazole ultraviolet absorbers in artificial leathers available commercially.UV-P and UV-329 at different content levels were detected in six samples.