基于硫化镍的C-反应蛋白免疫传感器的研制

本文合成了负载金纳米颗粒(Au NPs)的NiS纳米材料,通过壳聚糖(CHIT)将其固定在玻碳电极表面作为电化学生物传感器的固定基质。将C-反应蛋白(CRP)抗体固定到修饰过的玻碳电极表面,利用二茂铁甲酸标记CRP抗体,构建夹心型CRP生物传感器。采用差分脉冲伏安法(DPV)检测标记物二茂铁甲酸在0.3V左右的特征峰信号,该电流与培育的CRP抗原量成正比,从而实现对CRP的定量检测。传感器检测CRP的线性范围为0.01~500ng/mL,线性相关系数为0.9939,检测限为3.3pg/mL。

Preparation of C-reactive Protein Immunousensor Based on Nickel Sulfide Material

In this article,nickel sulfide was synthesized and used to load gold nanoparticles (Au NPS) and modified on the surface of glassy carbon electrode by chitosan as immobilized matrix to fix C-reactive protein antibody(anti-CRP).Ferroennecarboxylic acid was labeled on the anti-CRP to fabricate sandwich type of CRP immunosensor.Differential pulse voltammetry was used to detect a reduction signal peak of ferrocenecarboxylic acid at 0.3 V which is proportional to the concentration of CRP antigen.Under the optimal experimental conditions,the linear range was 0.01-500 ng/mL with a detection limit of 3.3 pg/mL.This immunosensor provides a method for effective and sensitive detection of CRP.