生物/化学同步聚合法制备纤维蛋白纳米复合物及传感研究

本文以有效提高生物分子包埋率为目的,基于生物/化学同步聚合的新方法制备了一种新型纤维蛋白聚合物基纳米复合物,并研究了该复合物修饰电极的传感性能。该方法在凝血仿生聚合的同时,采用NaAuCl4作为氧化剂化学氧化聚合生成聚多巴胺(PDA),在PDA膜内原位合成纳米金(AuNPs),同时在PDA-纤维蛋白凝胶生长时包埋葡萄糖氧化酶(GOx)。生物/化学同步聚合法操作简单,条件温和。该纳米复合物引入了AuNPs的优异性质,有效提升了GOx的包埋量,所制电化学生物传感器对葡萄糖的检测灵敏度高达117μA/(cm2·mmol/L),检测限为57nmol/L。

Biochemical/Chemical Synchronous Polymerization and Biosensing Applications

In order to improve the efficiency of biological molecular encapsulation,we propose a novel synchronous polymerization protocol based on biological coagulation polymerization and chemical oxidation polymerization.Novel fibrin-polymer-enzyme-gold nanoparticles bionanocomposites were prepared for fabricating high performance glucose biosensor.The protocol generated gold nanoparticles in polydopamine membrane and in situ entrapped plenty of glucose oxidase(GOx).This one-pot process was simple and mild.In addition,the novel protocol introduced the excellent properties of AuNPs,which effectively enhanced the immobilization efficiency and kept the activity of GOx.The fabricated electrochemical biosensor exhibited high sensitivity(117 cm2 · mmol/L) and low detection limit(57 nmol/L)to glucose.