Group separation of ortho-PCBs, coplanar non-ortho-PCBs and PCDDs/PCDFs using an alumina column as an one-step clean-up procedure

 A novel, simple inexpensive and rapid clean-up procedure is presented for the separation and quantitative determination of polychlorinated biphenyls (ortho-PCBs), coplanar non-ortho-PCBs (PCB No. 77, 126, 169) and polychlorinated dibenzo-p-dioxins (PCDDs) and dibenzofurans (PCDFs) in environmental samples. This clean-up procedure is the first method separating ortho-PCBs, non-ortho-PCBs and PCDD/PCDFs in one step with a single activated alumina column. Firstly, the ortho-PCBs are eluted from the activated alumina with a non-polar solvent. The non-ortho-PCBs are isolated in the second fraction with a more polar solvent-mixture, and finally the PCDD/PCDFs are collected in a polar fraction. This clean-up procedure was used to determine the congener-specific concentrations of ortho-PCBs, non-ortho-PCBs 77, 126 and 169 and PCDD/PCDFs in two different seepage waters and in one sewage sludge by HRGC and HRMS. The toxic equivalent quantities (TEQs) of the individual PCDD/PCDF-congeners and some toxic coplanar PCB-congeners were estimated. Received: 26 June 1996/Revised: 11 September 1996/Accepted: 14 September 1996