三丁基锡化合物与DNA相互作用的光谱研究

通过紫外(UV)和圆二色(CD)光谱,研究了三丁基锡(TBT)与脱氧核糖核酸(DNA)的相互作用。结果表明,TBT具有双重作用。既作用于DNA的碱基部分,影响DNA双螺旋结构;又作用于DNA的磷酸基团,使构象发生变化。TBT的作用位点随时间也不同。短时间内作用,位点是DNA的碱基,紫外光谱表现为增色效应;长时间作用,位点是DNA的磷酸基团,紫外光谱表现为增色效应减弱。随着TBT浓度的增大,紫外光谱的增色效应增强。磷酸根离子浓度对TBT与DNA相互作用有影响。磷酸根的存在削弱了TBT与DNA间的相互作用,也表明TBT与DNA的作用是双重的。

Investigation on Interaction of Tributyltin Compound with DNA by Spectroscopy

The interaction of tributyltin(TBT) compound and DNA was studied by UV and CD spectrometry.The interaction shows as dual actions.TBT interacts not only with the base pairs,resulting in the change of the double spiral structure of DNA,but also with phosphorous groups of DNA,resulting in the conformation of DNA.The action sites of TBT at DNA vary with time.TBT acts on the base parts of DNA within a short time,which caused the hyperchromism effect in UV spectra,and on the phosphorous group of DNA in a long time,which caused hyperchromism effect in UV spectra decreasing.The hyperchromism effect decreases with the increase of TBT concentration.In addition,the phosphorous ions decrease the interaction of TBT and DNA,which is the evidence of the dual actions.