Determination of a new blood glucose-lowering agent in biological fluids by capillary gas chromatography using electron-capture detection |
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Authors: | V Uebis |
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Affiliation: | Boehringer Mannheim GmbH, Bioanalytical Department, Germany. |
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Abstract: | A gas chromatographic method for the determination of 5-(4-chlorophenyl)-2-[(4-methylphenyl)sulphonyl]-4-pentynoic acid (I) in plasma (serum) and urine has been developed. After an extraction process, the cleaned-up organic extract was derivatized with diazomethane at ambient temperature. Results are evaluated from peak-height ratios with respect to the appropriate internal standard. The detection limit following extraction of a 1-ml plasma sample is about 20 ng/ml. |
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