Determination of rutin and forsythin in fruit ofForsythia suspensa(Thunb.) Vahl by capillary electrophoresis-electrochemical detection |
| |
Authors: | Xiangjun Li Yuping Zhang Zhuobin Yuan |
| |
Institution: | (1) Department of Chemistry, Graduate School, USTC, Chinese Academy of Sciences, 100039 Beijing, P.R. China |
| |
Abstract: | Summary Capillary electrophoresis-amperometric detection is evaluated for simultaneous determination of rutin and forsythin. The cyclic
voltammogram, hydrodynamic voltammogram, effect of pH, buffer concentration and SDS, and percent organic modifier on separation
and detection were studied. Conditions were optimized as follows: 1.2 V detection potential; separation at 12 kV; 5 s at 15
kV for sample injection time and sample injection voltage; mobile phase 20 mM boric acid buffer; pH 8.4, containing 40 mM
SDS and 10% (v/v) acetontrile. The method gave low detection limit as 0.001 mg mL−1 and 0.0005 mg mL−1 (S/N=3), wide linear range 0.005–0.5 mg mL−1 for rutin and forsythin, respectively. The relative standard deviations of peak current and migration time for 8 consecutive
injections of the standard solution containing 0.1 mg mL−1 each compound were 4.78%, 3.63% and 6.40%, 2.95% for rutin and forsythin, respectively. In addition, levels of the two compounds
in traditional Chinese herbal drugs were easily determined. |
| |
Keywords: | Capillary electrophoresis Amperometric detection Chinese herbal drugs Rutin Forsythin |
本文献已被 SpringerLink 等数据库收录! |
|