PICA法制备用于高效液相色谱的锆胶基质柱填料

迄今 ,硅胶基质固定相在 HPLC领域仍占据主导地位 ,但由于它的 p H使用范围窄 ,尤其在碱性条件下基质逐渐溶解 ,其使用受到限制 [1] .为此 ,寻找稳定性能高的新基质成为当前色谱学研究的热点之一 ,二氧化锆因具有良好化学稳定性和机械强度而受到关注 .目前 ,制备微米级、球形和多孔二氧化锆基质柱填料的常用方法有两种 :(1 )油乳化法 (OEM) ;(2 )聚合诱导胶体凝聚法 (PICA) .OEM法操作简单 ,但制备的二氧化锆微球粒径分布宽且孔径较小 .Carr[2 ,3] ,Unger[4 ] ,Rassi[5,6 ]和 Kawahara等[7,8] 在用 OEM法制备二氧化锆微球方面做了…

Preparation of Zirconia by PICA Method for HPLC

The methods for producing inorganic particles with a narrow particle size distribution, which are useful as chromatographic packing materials for high performance liquid chromatography(HPLC), are provided.These methods are based on improvements in the polymerization induced colloid aggregation(PICA) process described.These improved methods involved both synthesis of zirconium oxide colloid and sintering method.Colloid is an important factor in the PICAprocess.The reaction factors of the aqueous sol colloid of zirconium oxide are studied.We use sintering procedure to sinter zirconium oxide colloid, the average particle size is 5μm.Owing to different sintering procdure, the average pore size is between7.0_32 nm and the average surface area is between44_87 m²/g