Combined capillary gas chromatography/ion trap mass spectrometry quantitative methods using labeled or unlabeled internal standards |
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Authors: | R. J. Strife J. R. Simms M. P. Lacey |
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Affiliation: | 1. Corporate Research Division, Miami Valley Laboratories, The Procter & Gamble Company, 45239-8707, Cincinnati, Ohio, USA
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Abstract: | Three methods of operating an ion trap mass spectrometer (ITMS) were investigated for the determination of quantitative information by combined capillary GC/MS and GC/MS/MS. Separate regression Lines for a hexynone drug were examined by using bath unlabeled and stable-isotope-labeled internal standards. The ITMS modes of operation examined were (1) the full-scan, rf-voltage-only mode, useful on ion trap detectors as well as on the ITMS, (2) a scan using combined rf and dc voltages (rf/dc) for mass-selective storage analyses, and (3) rf/dc followed by collision-induced dissociation, Results of over 200 analyses in the 0.1–10 ng on-column range, with 5 ng of internal standard, showed that the unlabeled internal standard, separated by retention time on the capillary GC column, gave the best relative standard deviatiod (less than 5% over the range) and linear correlations (r 2 typically > 0.9992). |
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