In situ synthesis of DNA micro-arrays using typography technique |
| |
Authors: | Tang JianXin He NongYue and Li Song |
| |
Institution: | (1) State Key Laboratory of Bioelectronics, Southeast University, Nanjing, 210096, China;(2) Key Laboratory of Green Packaging and Application of Biological Nanotechnology of Hunan Province, Hunan University of Technology, Zhuzhou, 412008, China;(3) Zhongshan Huoju Polytechnic, Zhongshan, 528436, China |
| |
Abstract: | A novel typography technique was developed to in situ synthesize oligonucleotide arrays on glass slide, which has the celerity, high spatial resolution, lower cost, reliable operation,
and high synthetic efficiency. The principle and process of the typography technique for fabricating gene-chips have been
described in detail. A suit of poly(terafluoroethylene) devices for synthesizing oligonucleotide arrays were designed and
prepared, and the fiber tubes with a number of nano-or micron-channels were employed. The oligonucleotide arrays of 16 and
160 features with four different probes were synthesized using the typography technique. The four specific oligonucleotide
probes including the matched and the mismatched by the fluorescent target sequence gave obviously different hybridization
fluorescent signals. It was indicated that the gene-chip fabricated by the typography method could be used to rapidly screen
single-nucleotide polymorphisms (SNP) and to detect mutations.
Supported by the National Natural Science Foundation of China (Grant Nos. 60571032, 60571001, 90606027 and 60121101), the
National Hi-Tech Research and Development Program of China (Grant No. 2006AA03Z357), the Natural Science Foundation of Hunan
Province (Grant Nos. 04jj40023 and 06JJ4012) and the Natural Science Foundation of Guangdong Province (Grant No. 04008782) |
| |
Keywords: | typography oligonucleotide in situ synthesis DNA microarrays |
本文献已被 SpringerLink 等数据库收录! |
|