Lateral spread of an amplification signal using an enzymatic system on a conductive surface |
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Authors: | Hasenbank Melissa S Fu Elain Yager Paul |
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Affiliation: | University of Washington, Department of Bioengineering, Box 355061, Seattle, Washington 98195, USA. hasenm@u.washington.edu |
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Abstract: | In this letter, we report a novel signal amplification phenomenon that rapidly and dramatically increases both the magnitude and the lateral extent of the original signal. This phenomenon utilizes an enzyme immobilized on a conductive surface to generate amplified signals at locations remote from the original site of enzyme activity. The result is demonstrated on a microfluidic platform using the established precipitating enzyme-substrate system of horseradish peroxidase (HRP) and 3,3',5,5'-tetramethylbenzidine (TMB) on a surface plasmon resonance (SPR) imaging system. |
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