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| 地表水微囊藻毒素的表面等离波子共振免疫检测方法研究 | |
| 引用本文： | 王晓萍,詹舒越,罗昭锋,周宏敏.地表水微囊藻毒素的表面等离波子共振免疫检测方法研究[J].光学学报,2012,32(2):212005-155. |
| 作者姓名： | 王晓萍 詹舒越 罗昭锋 周宏敏 |
| 作者单位： | 王晓萍:浙江大学现代光学仪器国家重点实验室, 浙江 杭州 310027 詹舒越:浙江大学现代光学仪器国家重点实验室, 浙江 杭州 310027 罗昭锋:中国科学技术大学合肥微尺度国家实验室, 安徽 合肥 230027 周宏敏:中国科学技术大学合肥微尺度国家实验室, 安徽 合肥 230027 |
| 基金项目： | 国家863计划（2009AA06Z406）、国家自然科学基金重点项目（61036012）和中央高校基本科研业务费专项资助课题。 |
| 摘 要： | 结合表面等离波子共振(SPR)技术与免疫检测技术,研究和建立了一种响应速度快、免标记、低成本的地表水微囊藻毒素(MC-LR)检测方法。基于SpreetaTM传感器构建了小型SPR免疫检测系统,采用共价偶联方法在传感器金膜表面修饰MC-LR-BSA抗原为生物敏感膜;开展了MC-LR的SPR免疫检测方法实验研究,结果表明该方法的相对标准偏差为1.0%(n=6),定量范围为2～32ng/mL,检测限为0.63ng/mL,半抑制浓度CI50为10.7ng/mL,空白加标回收率和样品加标回收率在90%～113%之间。实样检测实验中,管道末梢饮用自来水水样未能检测出MC-LR,而某湖水水样中MC-LR的质量浓度为2.46ng/mL。实验研究与结果表明,MC-LR的SPR免疫检测方法可以满足世界卫生组织(WHO)对于饮用水中MC-LR最低含量检测的需求。 |
| 关 键 词： | 测量 表面等离波子共振 水质检测 微囊藻毒素MC-LR 免疫检测方法 |
| 收稿时间： | 2011/8/8 |
Research of Surface Plasmon Resonance Immunoassay Method for Microcystin-LR in Surface Water |
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| Wang Xiaoping,Zhan Shuyue,Luo Zhaofeng,Zhou Hongmin.Research of Surface Plasmon Resonance Immunoassay Method for Microcystin-LR in Surface Water[J].Acta Optica Sinica,2012,32(2):212005-155. | |
| Authors: | Wang Xiaoping Zhan Shuyue Luo Zhaofeng Zhou Hongmin |
| Institution: | 1 State Key Laboratory of Modern Optical Instrumentation,Zhejiang University,Hangzhou,Zhejiang 310027,China 2 Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences,University of Science and Technology of China,Hefei,Anhui 230027,China |
| Abstract: | A novel assay detection method for microcystin-LR (MC-LR) in surface water is researched and established which combines surface plasmon resonance (SPR) technique with immunoassay method. SpreetaTM sensor is adopted in the SPR flow injection analysis (FIA) system. The reusable biosensor chip is functionalized with (MC-LR)-BSA using covalent-coupling method. Experimental studies of SPR immunoassay method for MC-LR are carried out, the results show that relative standard deviation (RSD), quantitative range, detective limit and half maximal inhibitory concentration (CI50) are 1.0% (n=6), 2~32 ng/mL, 0.63 ng/mL and 10.7 ng/mL,respectively. Recovery rate of blank sample and different water samples spiked MC-LR ranges from 90% to 113%. It is concluded that tap water does not contain MC-LR while pond water contains 2.46 ng/mL MC-LR in real sample assay. The experimental results demonstrate that the SPR immunoassay can well meet drinking water guidelines of the World Health Organization (WHO) for MC-LR. |
| Keywords: | measurement surface plasmon resonance water-quality detection microcystin-LR immunoassay method |
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