|
|||||
|
|
Enzymatic microreactors for the determination of ethanol by an automatic sequential injection analysis system |
|
| Authors: | Alhadeff Eliana M Salgado Andrea M Cos Oriol Pereira Nei BelkisValdman Valero Francisco |
| Institution: | 1.Escola de Química, Centro de Tecnologia, Universidade Federal do Rio de Janeiro, Ilha do Fund?o, Cidade Universitária, CEP: 21.949-900, Rio de Janeiro, Brasil ;2.Department d’Enginyeria Química, Escola Tècnica Superior d’Enginyeria, Universitat Autònoma de Barcelona, 08193, Bellaterra, Barcelona, Spain ; |
| Abstract: | A sequential injection analysis system with two enzymatic microreactors for the determination of ethanol has been designed. Alcohol oxidase and horseradish peroxidase were separately immobilized on glass aminopropyl beads, and packed in 0.91-mL volume microreactors, working in line with the sequential injection analysis system. A stop flow of 120 s was selected for a linear ethanol range of 0.005-0.04 g/L +/- 0.6% relative standard deviation with a throughput of seven analyses per hour. The system was applied to measure ethanol concentrations in samples of distilled and nondistilled alcoholic beverages, and of alcoholic fermentation with good performance and no significant difference compared with other analytical procedures (gas chromatography and high-performance liquid chromatography). |
| Keywords: | |
| 本文献已被 PubMed SpringerLink 等数据库收录！ | |