| 乙肝病毒聚合酶链反应扩增产物的毛细管电泳测定 |
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| 引用本文： | 林炳承 刁海燕. 乙肝病毒聚合酶链反应扩增产物的毛细管电泳测定[J]. 分析化学, 1994, 22(5): 445-452 |
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| 作者姓名： | 林炳承 刁海燕 |
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| 作者单位： | 中国科学院大连化学物理研究所!大连116012(林炳承，张岩，褚新华)，大连市卫生防疫站(刁海燕) |
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| 基金项目： | 国家自然科学基金资助项目 |
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| 摘 要： | 本文用毛细管电泳对乙肝病毒聚合酶反应扩增产物进行了检测，并用这一方法和酶联免疫吸附实验以及ＰＣＲ与传统电泳联用的方法进行比较，探讨了用高效，快速，微量，易自动化的毛细管电泳代替传统检测方法的可能性，取得了令人满意的结果。
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| 关 键 词： | 乙型肝炎病毒 ELISA 毛细管电泳 |
Determination of Polymerase Chain Reaction-Amplified Products of Hepatitis B Virus With Non-gel Sieving Capillary Electrophoresis |
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| Lin Bingcheng,Diao Haiyan,Zhang Yan,Chu Xinhua. Determination of Polymerase Chain Reaction-Amplified Products of Hepatitis B Virus With Non-gel Sieving Capillary Electrophoresis[J]. Chinese Journal of Analytical Chemistry, 1994, 22(5): 445-452 |
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| Authors: | Lin Bingcheng Diao Haiyan Zhang Yan Chu Xinhua |
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| Abstract: | Non-gel sieving mode of capillary electrophoresis (CE) recommended by Hjerten and Zhu was used to determine the polymerase chain reaction (PCR) product of the hepatitis B virus. The 565 bp fragment of DNA of the hepatitis B virus was checked within only 12 min. Quantitative analysis of the fragments is also possible. Several samples of the patients were measured and the results were compared with those got from PCR-conventional electrophoresis and those from enzyme linked immunosorbent assay. Because the extent of sieving depends on the viscosity of the medium and on the chain length of the polymer , the selectivity will be flexible according to the use of the medium and/or the polymer. |
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| Keywords: | Hepatitis B virus Polymerase chain reaction Capillary electrophoresis |
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