微流控芯片分析及其在酶抑制剂筛选中的应用*

微流控芯片以其消耗少、易于微型化和集成化等优点在酶分析领域占有重要地位。近年来随着新检测技术的不断出现,酶抑制剂筛选芯片的结构也从简单的“混合-反应”和“分离-检测”,变得更加多样化和多功能化。微流控芯片上分子固定化酶、细胞培养等技术的进步为微流控芯片上实现酶抑制剂的高通量和高内涵筛选带来了巨大优势。本文对用于酶分析的微流控芯片的种类和构型进行简介和归纳总结,重点讨论和综述了其在酶抑制剂筛选中的应用及其最新研究进展。

Microfluidic chip and its application in enzyme inhibitor screening*

Microfluidic chip plays an important role in enzyme domain analysis because of its advantages of less consumption, easy miniaturization, integration and so on. In recent years, with the developments of methodology of cell culture on chip and molecular enzyme immobilization, high throughput and high content screening conditions on a microfluidic chip for enzyme inhibitors are proposed and developed rapidly. Furthermore, the structure of enzyme inhibitor screening chips becomes more diversified and more functional instead of the simple "mixed-reaction" and "separation -detection". The different types and configurations of microfluidic enzymatic analysis chips was introduced and summarized in this paper and the application in enzyme inhibitor screening and the latest progress of microfluidic enzymatic analysis were mainly discussed and reviewed.